plasmid addgene plasmid Search Results


6290 bp reporter plasmid pgfpns  (Addgene inc)
90
Addgene inc 6290 bp reporter plasmid pgfpns
6290 Bp Reporter Plasmid Pgfpns, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6290 bp reporter plasmid pgfpns/product/Addgene inc
Average 90 stars, based on 1 article reviews
6290 bp reporter plasmid pgfpns - by Bioz Stars, 2026-06
90/100 stars
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plenti puro  (Addgene inc)
94
Addgene inc plenti puro
Plenti Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti puro/product/Addgene inc
Average 94 stars, based on 1 article reviews
plenti puro - by Bioz Stars, 2026-06
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inducible nil cassette  (Addgene inc)
93
Addgene inc inducible nil cassette
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Inducible Nil Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inducible nil cassette/product/Addgene inc
Average 93 stars, based on 1 article reviews
inducible nil cassette - by Bioz Stars, 2026-06
93/100 stars
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pcdna3 1 mycbioid  (Addgene inc)
94
Addgene inc pcdna3 1 mycbioid
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Pcdna3 1 Mycbioid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1 mycbioid/product/Addgene inc
Average 94 stars, based on 1 article reviews
pcdna3 1 mycbioid - by Bioz Stars, 2026-06
94/100 stars
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plex307 ace2 puro  (Addgene inc)
93
Addgene inc plex307 ace2 puro
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Plex307 Ace2 Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plex307 ace2 puro/product/Addgene inc
Average 93 stars, based on 1 article reviews
plex307 ace2 puro - by Bioz Stars, 2026-06
93/100 stars
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pl sin eos s 4 eip  (Addgene inc)
93
Addgene inc pl sin eos s 4 eip
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Pl Sin Eos S 4 Eip, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pl sin eos s 4 eip/product/Addgene inc
Average 93 stars, based on 1 article reviews
pl sin eos s 4 eip - by Bioz Stars, 2026-06
93/100 stars
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addgene plasmid  (Addgene inc)
92
Addgene inc addgene plasmid
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/addgene plasmid/product/Addgene inc
Average 92 stars, based on 1 article reviews
addgene plasmid - by Bioz Stars, 2026-06
92/100 stars
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pax3 foxo1  (Addgene inc)
93
Addgene inc pax3 foxo1
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Pax3 Foxo1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pax3 foxo1/product/Addgene inc
Average 93 stars, based on 1 article reviews
pax3 foxo1 - by Bioz Stars, 2026-06
93/100 stars
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hif 1 protein hif 1 responsive luciferase construct  (Addgene inc)
94
Addgene inc hif 1 protein hif 1 responsive luciferase construct
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Hif 1 Protein Hif 1 Responsive Luciferase Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hif 1 protein hif 1 responsive luciferase construct/product/Addgene inc
Average 94 stars, based on 1 article reviews
hif 1 protein hif 1 responsive luciferase construct - by Bioz Stars, 2026-06
94/100 stars
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n terminal his tag  (Addgene inc)
86
Addgene inc n terminal his tag
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
N Terminal His Tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n terminal his tag/product/Addgene inc
Average 86 stars, based on 1 article reviews
n terminal his tag - by Bioz Stars, 2026-06
86/100 stars
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plko cre stuffer v4 plasmid  (Addgene inc)
93
Addgene inc plko cre stuffer v4 plasmid
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Plko Cre Stuffer V4 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plko cre stuffer v4 plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
plko cre stuffer v4 plasmid - by Bioz Stars, 2026-06
93/100 stars
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eric kowarz  (Addgene inc)
93
Addgene inc eric kowarz
Direct programming is an efficient and robust protocol for the generation <t>of</t> <t>MNs</t> from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 <t>(NIL)</t> cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.
Eric Kowarz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eric kowarz/product/Addgene inc
Average 93 stars, based on 1 article reviews
eric kowarz - by Bioz Stars, 2026-06
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Image Search Results


Direct programming is an efficient and robust protocol for the generation of MNs from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 (NIL) cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.

Journal: NAR Molecular Medicine

Article Title: Neuromuscular dysfunction in patient-derived FUS R244RR –ALS iPSC model via axonal downregulation of neuromuscular junction proteins

doi: 10.1093/narmme/ugaf005

Figure Lengend Snippet: Direct programming is an efficient and robust protocol for the generation of MNs from hiPSCs and hESCs. ( A ) A scheme of the homology mediated repair template for insertion of NGN2-ISL1-LHX3 (NIL) cassette into CLYBL genetic locus. CbA: chicken β-actin; SA: splice acceptor; HA L/R: homology arm left/right; Neo: neomycin resistance gene. ( B ) Differentiation protocol time course with exemplary images (Hues-3 line) illustrating morphological changes of the cells; DOX: doxycycline; iMN: human-induced MN; scale bar: 100 μm. ( C ) iMNs express neuronal (TUBB3, cyan), dendritic (MAP2, yellow), and axonal (NF, magenta) markers. iMNs derived from hiPSC-NIL (left) and hESC-NIL-HB9::GFP (right) on day 7 are shown; scale bar: 20 μm. ( D and E ) Direct programming protocol produces a highly homogeneous (∼90%) MN culture. For the hiPSC-NIL differentiation, the percentage of HB9 positive cells and the exemplary IF images at day 7 are shown in panel (D). For hESC-NIL differentiation, the percentages of HB9 and GFP positive cells and the exemplary IF images at day 7 are shown in panel (E). HB9: magenta, GFP: yellow, DAPI: blue, scale bar: 20 μm.

Article Snippet: To generate cell lines for differentiation into induced MNs (iMNs), the construct with the inducible NIL cassette [ ] (Addgene #105841) was transfected into these lines using Lipofectamine 3000 and stably inserted into using TALENs (Addgene #62197 and #62196).

Techniques: Derivative Assay